Crystal Research and Technology
Cryst. Res. Technol. 41, 1063 (2006) - Abstract -

Sedimentation as a tool for crystallization from protein mixtures

I. Dimitrov and C. N. Nanev

Rostislaw Kaischew Institute of Physical Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

Keywords protein crystallization, apoferritin, lysozyme, zonal sedimentation, free interface diffusion
PACS 87.14.Ee, 87.15.Nn
DOI 10.1002/crat.200610722

Crystals from apoferritin which is an iron-free form of protein ferritin were obtained from protein mixtures lysozyme/apoferritin using sedimentation under high gravity. Solution containing apoferritin at concentration as high as 5mg/ml in the presence of 25mg/ml lysozyme and overlaid on 5%(w/v) CdSO4 in 0,2M/L NaAC, pH=5 still favors apoferritin crystal formation under normal gravity conditions, but at apoferritin concentrations <0,5mg/ml (~1,14μM/L) in 25mg/ml (~1,71mM/L) lysozyme only the sedimentation in a centrifuge appears to be useful for separating the apoferritin molecules from the mixture followed by apoferritin crystallization in the same system. The very high molecule number ratio (~1:1000) of two proteins is used to stress on the observed effect.





The full text of this paper in pdf-Format:

If you have come directly to this page, click this symbol to go to the CRT homepage.