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Crystal Research and Technology |
Cryst. Res. Technol. 41, 1063 (2006) - Abstract -
Sedimentation as a tool for crystallization from protein mixtures
I. Dimitrov and C. N. Nanev
Rostislaw Kaischew Institute of Physical Chemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
| Keywords | protein crystallization, apoferritin, lysozyme, zonal sedimentation, free interface diffusion |
| PACS | 87.14.Ee, 87.15.Nn |
| DOI | 10.1002/crat.200610722 |
Crystals from apoferritin which is an iron-free form of protein ferritin were obtained from protein mixtures lysozyme/apoferritin using sedimentation under high gravity. Solution containing apoferritin at concentration as high as 5mg/ml in the presence of 25mg/ml lysozyme and overlaid on 5%(w/v) CdSO4 in 0,2M/L NaAC, pH=5 still favors apoferritin crystal formation under normal gravity conditions, but at apoferritin concentrations <0,5mg/ml (~1,14μM/L) in 25mg/ml (~1,71mM/L) lysozyme only the sedimentation in a centrifuge appears to be useful for separating the apoferritin molecules from the mixture followed by apoferritin crystallization in the same system. The very high molecule number ratio (~1:1000) of two proteins is used to stress on the observed effect.

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